- 作者：Ana Carolina Ganime ; Filipe A. Carvalho-Costa ; Marcos Cesar L. Mendon?a ; Carmen B. Vieira ; Marisa Santos ; Rubens Costa Filho ; Marize P. Miagostovich ; Jos¨¦ Paulo G. Leite
- 关键词：Fomites ; Virus biomarker ; Hospital infection ; Adult ward
- 刊名：American Journal of Infection Control
- 出版年：2012
- 出版时间：August, 2012
- 年：2012
- 卷：40
- 期：6
- 页码：544-547
- 全文大小：127 K
Background
Environmental surfaces can play a role in the spread of pathogens, such as enteric viruses, within a hospital. This study assessed the level of contamination of group A rotavirus (RV-A) on environmental surfaces samples from an adult intensive care unit in a hospital in Rio de Janeiro, Brazil.Methods
A total of 504 environmental surface samples were obtained from multiple sites in the intensive care unit, including flushing buttons, telephones, and alcohol gel supports. Nested and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) were used to detect and quantify RV-A levels through partial amplification of VP6 and NSP3 genes, respectively, and the viability of the viruses detected was assessed by MA-104 cell integrated cell culture/RT-PCR.
Results
RV-A was detected by nested RT-PCR in 14 % of the samples (73 of 504), with viral loads ranging from 3.4 genomic copies/mL to 2.9?¡Á 103 genomic copies/mL. The nucleotide sequence of the amplicons obtained from nested RT-PCR confirmed that the positive samples were RV-A. Moreover, 3 of 10 strains investigated demonstrated viability by integrated cell culture/RT-PCR.
Conclusion
The detection of RV-A on environmental surface samples indicates a need for improvements to hospital cleaning procedures to reduce viral contamination, and suggests, as reported previously, that RV-A can be used as a biomarker to assess contamination in hospitals.