Di 2-ethyl hexyl phthalate affects differentiation and matrix mineralization of rat calvarial osteoblasts - in vitro
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- 作者：Firdous Ahmad Bhat ; G. Ramajayam ; S. Parameswari ; R.C. Vignesh ; S. Karthikeyan ; K. Senthilkumar ; G.D. Karthikeyan ; K. Balasubramanian ; J. Arunakaran ; N. Srinivasan
- 关键词：ALP ; alkaline phosphatase ; BPA ; bisphenol A ; BSP ; bone sialoprotein ; CDK ; cyclin dependent kinase ; DEHP ; di 2-ethyl hexyl phthalate ; DIDP ; diisodecyl phthalate ; ED ; endocrine disruptor ; OPN ; osteopontin ; PCB ; polychlorinated biphenyls ; ROB ; rat calvarial
- 刊名：Toxicology in Vitro
- 出版年：2013
- 出版时间：February, 2013
- 年：2013
- 卷：27
- 期：1
- 页码：250-256
- 全文大小：1000 K
文摘
Di-2-ethyl hexyl phthalate (DEHP), an industrial plasticizer and a ubiquitous environmental contaminant, is an established endocrine disruptor (ED). Increasing evidences indicate that some EDs interfere with osteoblast differentiation and function. In the present study, we investigated the effects of DEHP on the expression of cell cycle proteins, differentiation markers, Runx2 and its co-activator TAZ in osteoblasts derived from neonatal rat calvaria. A significant decrease in protein levels of cyclin D1 and CDK-2 was found at high dosage of DEHP (100 ¦ÌM) after 24 h treatment. DEHP treatment caused a significant decrease in ALP mRNA. While DEHP treatment significantly decreased the TAZ at mRNA and protein levels, it decreased only the Runx2protein levels. Histochemical localization of ALP, collagen and mineralized nodules studied from cells treated with DEHP (10 and 100 ¦ÌM) for 21 days revealed a drastic decrease in collagen, ALP and mineralization. In conclusion, DEHP affected differentiation of neonatal rat calvarial osteoblasts and mineralization of matrix secreted by these cells.