Interaction between coat protein and replication initiation protein of Mung bean yellow mosaic India virus might lead to control of viral DNA replication

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• 作者：Malik ; Punjab Singh ; Kumar ; Vikash ; Bagewadi ; Basavaraj ; Mukherjee ; Sunil K.
• 关键词：MYMIV ; DNA replication ; Rolling circle replication (RCR) ; Rep ; CP ; Pull-down ; Yeast two-hybrid ; Oligomerization ; Nicking and closing
• 刊名：Virology
• 出版年：2005
• 出版时间：July 5, 2005
• 年：2005
• 卷：337
• 期：2
• 页码：273-283
• 全文大小：476 K

文摘

In addition to their encapsidation function, viral coat proteins (CP) contribute to viral life cycle in many different ways. The CPs of the geminiviruses are responsible for intra- as well as inter-plant virus transmission and might determine the yield of viral DNA inside the infected tissues by either packaging the viral DNA or interfering with the viral replicative machinery. Since the cognate Rep largely controls the rolling circle replication of geminiviral DNA, the interaction between Rep and CP might be worthwhile to examine for elucidation of CP-mediated control of the viral DNA copy number. Here a reasonably strong interaction between Rep and CP of the geminivirus Mung bean yellow mosaic India virus is reported. The domain of interaction has been mapped to a central region of Rep. The replication initiation activity of Rep, i.e., its nicking and closing function, is down regulated by CP. This report highlights how CP could be important in controlling geminiviral DNA replication.