Enhancing of plasmonic photothermal therapy through heat-inducible transgene activity

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• 作者：Virginia Cebriá ; n ; BS^a ; ^b ; Francisco Martí ; n-Saavedra ; PhD^a ; ^b ; Leyre Gó ; mez ; BS^c ; Manuel Arruebo ; PhD^a ; ^c ; Jesus Santamaria ; PhD^a ; ^c ; Nuria Vilaboa ; PhD^a ; ^b ; ^{nvilaboa.hulp@salud.madrid.org}
• 关键词：Nanoshells ; Hollow gold nanoparticles ; Photothermal therapy
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：9
• 期：5
• 页码：646-656
• 全文大小：4969 K

文摘

We explore the synergistic effect of photothermal therapy and gene therapy, simultaneously triggered by silica-gold nanoshells (NS) or hollow gold nanoparticles (HGNPs) in human HeLa cells following near-infrared (NIR) light irradiation. Thermal transfer from NS was higher than that displayed by HGNPs, owing to a differential interaction of the nanomaterial with the biological environment. Under sublethal photothermal conditions, NS and HGNPs effectively modulated the expression levels of a DsRed-monomer reporter gene controlled by the highly heat-inducible human HSP70B promoter, as a function of nanomaterial concentration and length of laser exposure. Hyperthermia treatments at doses that do not promote cell death generated a lethal outcome in HeLa cells harboring the fusogenic GALV-FMG transgene under the control of the HSP70B promoter. Combination of lethal photothermia with the triggering of the cytotoxic transgene resulted in a dramatic increase of the cell-ablation area as a result of the synergistic activity established.

From the Clinical Editor

In this study photothermal therapy and gene therapy, simultaneously triggered by silica-gold nanoshells or hollow gold nanoparticles, was investigated in human HeLa cells following near-infrared (NIR) light irradiation. It is shown that the combination of lethal photothermia with the triggering of the cytotoxic transgene at sublethal levels results in a synergistic cytotoxic effect in vitro.