Functionalization of 3D scaffolds with protein-releasing biomaterials for intracellular delivery
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- 作者：Joaquin Seras-Franzoso ; Christoph Steurer ; M¨°nica Rold¨¢n ; Meritxell Vendrell ; Carla Vidaurre-Agut ; Anna Tarruella ; Laura Salda?a ; Nuria Vilaboa ; Marc Parera ; Elisa Elizondo ; Imma Ratera ; Nora Ventosa ; Jaume Veciana ; Alberto J. Campillo-Fern¨¢ndez ; Elena Garc¨ªa-Fruit¨®s ; Esther V¨¢zquez ; Antonio Villaverde
- 关键词：Nanoparticles ; 3D scaffolds ; Polylactic acid (PLA) ; Bottom-up delivery ; Bioscaffold ; Tissue engineering
- 刊名：Journal of Controlled Release
- 出版年：2013
- 出版时间：10 October, 2013
- 年：2013
- 卷：171
- 期：1
- 页码：63-72
- 全文大小：2188 K
文摘
Appropriate combinations of mechanical and biological stimuli are required to promote proper colonization of substrate materials in regenerative medicine. In this context, 3D scaffolds formed by compatible and biodegradable materials are under continuous development in an attempt to mimic the extracellular environment of mammalian cells. We have here explored how novel 3D porous scaffolds constructed by polylactic acid, polycaprolactone or chitosan can be decorated with bacterial inclusion bodies, submicron protein particles formed by releasable functional proteins. A simple dipping-based decoration method tested here specifically favors the penetration of the functional particles deeper than 300 ¦Ìm from the materials' surface. The functionalized surfaces support the intracellular delivery of biologically active proteins to up to more than 80 % of the colonizing cells, a process that is slightly influenced by the chemical nature of the scaffold. The combination of 3D soft scaffolds and protein-based sustained release systems (Bioscaffolds) offers promise in the fabrication of bio-inspired hybrid matrices for multifactorial control of cell proliferation in tissue engineering under complex architectonic setting-ups.