Nephroprotective effects of (−)-α-bisabolol against ischemic-reperfusion acute kidney injury

详细信息    查看全文

• 作者：Tiago Lima Sampaio^a ; Ramon Ró ; seo Paula Pessoa Bezerra de Menezes^a ; ^{ramonppessoa@gmail.com} ; Marcus Felipe Bezerra da Costa^a ; Gdayllon Cavalcante Meneses^a ; Mauren Cristina Villalta Arrieta^b ; Adriano José ; Maia Chaves Filho^a ; Glayciane Bezerra de Morais^c ; Alexandre Braga Libó ; rio^d ; Renata Sousa Alves^e ; Janaí ; na Serra Azul Monteiro Evangelista^c ; Alice Maria Costa Martins^e
• 关键词：Ischemia/reperfusion ; Acute kidney injury ; (&minus ; )-α-Bisabolol ; KIM-1
• 刊名：Phytomedicine
• 出版年：2016
• 出版时间：15 December 2016
• 年：2016
• 卷：23
• 期：14
• 页码：1843-1852
• 全文大小：2244 K
• 卷排序：23

文摘

Ischemia/reperfusion (I/R) in kidney is commonly related to acute kidney injury (AKI), essentially through oxidative stress. (−)-α-Bisabolol is a sesquiterpene isolated from the essential oil of a variety of plants, including chamomile, which has important antioxidant activity.Study designThis study intends to evaluate the nephroprotective activity of (−)-α-bisabolol (Bis) in both in vivo and in vitro models of kidney I/R.MethodsMale Wistar rats were submitted to right nephrectomy, followed by ischemia by clamping of the renal artery in the left kidney for 60 min. and 48 h of reperfusion. The animals were treated orally with Bis (100 mg/kg) or vehicle for 24 h after reperfusion, and placed in metabolic cages, to evaluate water consumption, diuresis, urinary osmolality, classic biochemical markers and urinary KIM-1 (kidney injury molecule-1). Additionally, the left kidney was collected for histological evaluation and determination of glutathione (GSH) and Thiobarbituric Acid Reactive Substances (TBARS) levels. Tubular epithelial cells LLC-MK2 were used to assess Bis effect on in vitro I/R, by MTT assay. It was performed the cellular respiration tests by flow cytometry: evaluation of the production of cytoplasmic reactive oxygen species by DCFH-DA assay and mitochondrial transmembrane potential analysis with the dye rhodamine 123.ResultsI/R caused alterations in diuresis, water intake, urinary osmolality, plasmatic creatinine, urea and uric acid, creatinine clearance, proteinuria and microalbuminuria. Treatment with Bis ameliorated all of these parameters. Also, KIM-1 level enhanced by I/R was also diminished in groups treated with Bis. The histological examination showed that Bis attenuated the morphological changes caused by I/R, markedly vascular congestion and intratubular deposits of proteinaceous material. Additionally, Bis was able to reduce the changes observed in TBARS and GSH levels in kidney tissue. In in vitro assay, Bis was capable to partially protect the cell lineage against cell damage induced by I/R.Conclusion(−)-α-Bisabolol has a nephroprotective effect in kidney I/R, with antioxidant effect. Moreover, this result seems to be associated to a direct protective effect on tubular epithelia.