Amomum villosum induces longitudinal bone growth in adolescent female rats

详细信息    查看全文

• 作者：Sun Haeng Lee^a ; Ji Young Kim^b ; Hocheol Kim^b ; Seul Ki Park^a ; Cho Young Kim^a ; Sun Yong Chung^c ; Gyu Tae Chang^a ; ^{gtchang@khu.ac.kr}
• 关键词：Amomum villosum ; Growth plate ; Bone Development ; Recombinant human growth hormone
• 刊名：Journal of Traditional Chinese Medicine
• 出版年：2012
• 出版时间：September, 2012
• 年：2012
• 卷：32
• 期：3
• 页码：453-458
• 全文大小：1548 K

文摘

ass=""h4"">Objective

This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth.

ass=""h4"">Methods

Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days: control (distilled water, p.o.), recombinant human growth hormone (rhGH; 100 ¦Ìg/kg, s.c.), and A. villosum (500 mg/kg, p.o.) groups. On day 3, tetracycline (20 g/kg, i.p.) was injected for growth plate identification. On days 2, 3 and 4, 5-bromo-2¡ä-deoxyuridine (BrdU) (50 mg/kg, i.p.) was injected to label proliferating cells. On day 5, tibias were dissected and fixed in 4 % paraformaldehyde, dehydrated, and sectioned for immunohistochemistry and histomorphometry.

ass=""h4"">Results

The rate of bone growth in the A. villosum and rhGH groups increased to (410 ¡À 44) and (389 ¡À 46) ¦Ìm/day (P<0.01), respectively, as compared with the control (330.7 ¡À 34.7) ¦Ìm/day. The thickness of the growth plates also increased to (591 ¡À 37) and (598 ¡À 32) ¦Ìm, respectively, as compared with the control (524¡À89) ¦Ìm (P<0.001). The number of BrdU-positive cells in the chondrocytes of the A. villosum and rhGH groups was also significantly higher (126¡À24) and (143¡À18) cells/mm², respectively) than in the control (109 ¡À 25) mm² (P<0.05). Insulin-like growth factor-1 and bone morphogenetic protein-2 in the A. villosum and rhGH groups were highly expressed in the growth plate as compared with the control samples, indicating increased bone formation.

ass=""h4"">Conclusions

A. villosum could be used to treat growth retardation during adolescence.