Mitochondrial localization of the low level p53 protein in proliferative cells
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- 作者：Ioana Ferecatu ; Marie Bergeaud ; Aida Rodrí ; guez-Enfedaque ; Nathalie Le Floch ; Lisa Oliver ; Vincent Rincheval ; Flore Renaud ; Franç ; ois M. Vallette ; Bernard Mignotte ; Jean-Luc Vayssiè ; re
- 关键词：p53 ; Mitochondria ; VDAC ; Cell proliferation ; Apoptosis
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2009
- 出版时间：2 October 2009
- 年：2009
- 卷：387
- 期：4
- 页码：772-777
- 全文大小：1699 K
文摘
p53 protein plays a central role in suppressing tumorigenesis by inducing cell cycle arrest or apoptosis through transcription-dependent and -independent mechanisms. Emerging publications suggest that following stress, a fraction of p53 translocates to mitochondria to induce cytochrome c release and apoptosis. However, the localization of p53 under unstressed conditions remains largely unexplored. Here we show that p53 is localized at mitochondria in absence of apoptotic stimuli, when cells are proliferating, localization observed in various cell types (rodent and human). This is also supported by acellular assays in which p53 bind strongly to mitochondria isolated from rat liver. Furthermore, the mitochondria subfractionation study and the alkaline treatment of the mitochondrial p53 revealed that the majority of mitochondrial p53 is present in the membranous compartments. Finally, we identified VDAC, a protein of the mitochondrial outer-membrane, as a putative partner of p53 in unstressed/proliferative cells.