Reuse of anion exchangers as supports for enzyme immobilization: Reinforcement of the enzyme-support multiinteraction after enzyme inactivation
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- 作者：Jose J. Virgen-Ortí ; za ; 1 ; Sara Peircea ; b ; 1 ; Veymar G. Tacias-Pascacioa ; c ; Vicente Cortes-Corberana ; Antonio Marzocchellab ; Maria Elena Russod ; Roberto Fernandez-Lafuentea ; rfl@icp.csic.es" class="auth_mail" title="E-mail the corresponding author ; rfernandezlafuente@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ion exchange ; Reversibly immobilization ; Enzyme unfolding ; Support-enzyme multi-interaction ; Enzyme desorption ; Support reuse
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：51
- 期：10
- 页码：1391-1396
- 全文大小：1043 K
文摘
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β-Galactosidase from Aspergillus oryze has been immobilized on agarose beads coated with polyethyleneimine.
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Fresh enzyme could be fully desorbed using 500 mM NaCl at pH 7 and 25 °C.
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Inactivated preparations released the active molecules under similar conditions.
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Desorption of the inactivated enzyme required much more strong conditions.
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The fully clean support could be reused several cycles without lost performance.