To gain insights into mechanisms of PEDV-pAPN interactions, the present study aimed at identifying the domain that is critical for PEDV binding. Results showed PEDV infection was restricted to pAPN domain VII expressing NIH3T3 cells. PEDV harvested from pAPN or domain VII expressing NIH3T3 cells was induced indirect plaques in Vero cells. Our results demonstrate that PEDV recognizes pAPN and that the main interactive point is lodged within domain VII of the pAPN.