Analytical performance evaluation of the scanning capillary tube viscometer for measurement of whole blood viscosity

详细信息    查看全文

• 作者：Hanah Kim^a ; Young I. Cho^b ; Dong-Hwan Lee^c ; Chul-Min Park^a ; Hee-Won Moon^a ; Mina Hur^a ; Jin Q. Kim^a ; Yeo-Min Yun^a ; ^{ymyun@kuh.ac.kr}
• 关键词：CLSI ; Clinical Laboratory and Standards Institute ; cP ; centipoise ; CV ; coefficient of variation ; DBV ; diastolic whole blood viscosity ; mP ; milliPoise ; SBV ; systolic whole blood viscosity ; SCTV ; scanning capillary tube viscometer ; SD ; standard deviation ; W
• 刊名：Clinical Biochemistry
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：46
• 期：1-2
• 页码：139-142
• 全文大小：322 K

文摘

Background

Whole blood viscosity (WBV) is the resistance of blood flow in blood vessels. Increased WBV may be a cardiovascular risk factor. The proper screening of WBV can help the early detection of cardiovascular disease. We investigated the performance of a new scanning capillary tube viscometer (SCTV) for the measurement of WBV.

Methods

We evaluated the total precision of the SCTV for 20 days using three control viscosity materials, and the within-day precision with the whole blood samples of three different individuals. For the linearity evaluation, serial dilutions of a high concentration standard material were used. For the method comparison, the results of the SCTV method were compared to those of Brookfield rotating viscometer on 227 subjects.

Results

The SCTV had good within-run and total-run coefficient of variant (CV)s at low-, medium-, and high-concentration samples, at shear rates of 1 and 300 s^? 1. The within-day CVs with the three human blood samples were 6.3 % , 3.7 % and 3.8 % at a shear rate of 1 s^? 1, and 3.2 % , 3.0 % and 4.1 % at a shear rate of 300 s^? 1. The SCTV method showed an excellent linearity in the range of 84.9 to 558.2 milliPoise (mP) and 28.8 to 71.0 mP at shear rates of 1 and 300 s^? 1, respectively. For the comparison study, the SCTV and the rotating viscometer showed comparable results.

Conclusions

The SCTV showed a stable analytical performance, and was comparable with the rotational viscometer. This new SCTV method can be used in the clinical laboratory for various needs.