Gold nanoclusters-based dual-emission ratiometric fluorescence probe for monitoring protein kinase
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- 作者：Wei SongaAuthor Vitae ; Ru-Ping LiangaAuthor Vitae ; Ying WangaAuthor Vitae ; Li ZhangaAuthor Vitae ; Jian-Ding Qiua ; b ; jdqiu@ncu.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Ratiometric fluorescent ; Gold nanoclusters ; Carboxypeptidase Y ; Protein kinase ; Inhibitor
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：226
- 期：Complete
- 页码：144-150
- 全文大小：1785 K
文摘
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We reported a novel and visual ratiometric fluorescence assay for sensitive analysis of PKA activity.
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The peptide-AuNCs was synthesized in situ without any coupling and reducing agents.
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PKA assay was based on carboxypeptidase Y digestion quenching mechanism.
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Highly sensitive detection of PKA activity and inhibition was achieved.