A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection
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- 作者：Abkar Ahmed Sayada ; b ; Abkar8819@gmail.com" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Fatimah Ibrahima ; b ; fatimah@um.edu.my" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Shah Mukim Uddina ; bAuthor Vitae ; Koh Xiu PeicAuthor Vitae ; Mas S. Mohktara ; bAuthor Vitae ; Marc Madoua ; b ; d ; eAuthor Vitae ; Kwai Lin Thongb ; cAuthor Vitae
- 关键词：LAMP ; Pathogen ; Microfluidics ; Lab-on-a-disc ; Detection ; Salmonella
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：227
- 期：Complete
- 页码：600-609
- 全文大小：3089 K
文摘
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Development of a microfluidic compact disc (CD) device for foodborne pathogen detection.
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Prove of concept that loop mediated isothermal amplification (LAMP) can be done on centrifugal microfluidic CD platform.
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A technique to maintain constant temperature required for DNA amplification.
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Performed LAMP on microfluidic CD to detect Salmonella bacteria.
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Specificity and sensitivity of the LAMP assay on CD validated.