Development of a quantitative PCR for the detection of Rangelia vitalii
详细信息 查看全文
- 作者：Francine Chimelo Paima ; franchimelo@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Andrea Pires dos Santosb ; Naí ; la Cannes do Nascimentob ; Camila Serina Lastac ; Simone Tostes Oliveirad ; Joanne Belle Messickb ; Sonia Terezinha dos Anjos Lopesa
- 关键词：Quantitative PCR ; 18S rRNA gene ; Rangeliosis ; Dog
- 刊名：Veterinary Parasitology
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：217
- 期：Complete
- 页码：113-117
- 全文大小：861 K
文摘
- •
SYBR® Green qPCR was developed based on a fragment of the 18S rDNA of R. vitalii
- •
The assay was developed for detection/quantification of R. vitalii in dog’s blood
- •
The qPCR assay showed specificity and high sensitivity for detection of R. vitalii
- •
This assay was able to detect as few as one copy of R. vitalii
- •
qPCR assay developed herein is a suitable tool for detection of R. vitalii in dogs