A method for assessment of the genotoxicity of mainstream cigarette-smoke by use of the bacterial reverse-mutation assay and an aerosol-based exposure system
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- 作者：Joanne Kilforda ; David Thorneb ; Rebecca Paynea ; Annette Dalrympleb ; Julie Clementsa ; Clive Meredithb ; Debbie Dillonb ; Debbie_Dillon@bat.com" class="auth_mail
- 关键词：AAN ; 2-aminoanthracene ; B[a]P ; Benzo(a)pyrene ; DMSO ; dimethyl sulphoxide ; FI ; fold increase ; ISO ; international standards organisation ; MR ; mean revertants ; NaN3 ; sodium azide ; NQO ; nitroquinoline oxide ; PBS ; phosphate buffered saline ; PM ; particulate material ; QCM ; quartz crystal microbalance ; VC 10 ; Vitrocell® ; smoking robot ; VP ; vapour phase ; 2NF ; 2-nitrofluorene
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：15 July 2014
- 年：2014
- 卷：769
- 期：Complete
- 页码：20-28
- 全文大小：1153 K
文摘
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We have modified the Ames assay for the testing of a cigarette smoke aerosol.
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3R4F cigarette smoke was generated on the Vitrocell VC 10 Smoking Robot.
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Dose-related increases in revertants were observed in four strains of S. typhimurium.
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Quartz crystal microbalances measured particle deposition in situ for dosimetry purposes.
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The response to whole smoke in strain YG1042 was reproducible on a second VC 10.