Ectopic osteogenic capacity of freshly isolated adipose-derived stromal vascular fraction cells supported with platelet-rich plasma: A simulation of intraoperative procedure

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• 作者：Stevo J. Najman^a ; ^b ; ¹ ; ^{stevo.najman@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Vladimir J. Cvetković^c ; ¹ ; ^{biovlada@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; ^{vlad.cvet@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jelena G. Najdanović^a ; ^b ; ^{jella82@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Sanja Stojanović^a ; ^b ; ^{s.sanja88@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Marija Đ. Vukelić-Nikolić^a ; ^b ; ^{marijavukelic@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Ivica Vučković^d ; ^{drivicavuckovic@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Dragan Petrović^d ; ^e ; ^{dragan-petrovic@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ADSCs ; Adipose-derived SVF cells ; PRP ; Ectopic osteogenesis ; Bone-related genes
• 刊名：Journal of Cranio-Maxillofacial Surgery
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：44
• 期：10
• 页码：1750-1760
• 全文大小：4719 K

文摘

Bone defects represent a serious problem in cranio-maxillofacial surgery. Autologous adipose-derived stromal vascular fraction (SVF) cells in combination with biological factors and bone substitutes were previously proposed as alternative to bone grafting. By simulating an intraoperative procedure we examined osteogenic capacity of the combination of two autologous components, freshly isolated adipose-derived SVF cells, and platelet-rich plasma (PRP), delivered on bone mineral matrix (BMM) carrier (SPB group) in mice ectopic bone forming model. Implantation of BMM only (B group) was a control. The presence of adipose-derived stem cells (ADSCs) in SVF was detected by immunocytochemical analysis. Expression of bone- and endothelial-related genes was compared between freshly isolated SVF and ADSCs obtained from SVF after in vitro cultivation. The implants were analyzed using expression analysis of bone-related genes at one, two, four and eight weeks and histochemical, immunohistochemical and histomorphometrical analyses at two and eight weeks after implantation. Freshly isolated adipose-derived SVF contained ADSCs and exhibited promising osteogenic and vasculogenic capacity. At two and four weeks, significantly higher expression of bone-related genes was detected in SPB group compared to B group. The signs of osteogenic process were more pronounced in SPB than in B implants. By the end of experiment, percentage of infiltrated tissue and vascularization was significantly higher in SPB than in B implants. Adipose-derived SVF cells, PRP and BMM rapidly initiated osteogenesis what makes this combination promising candidate for treatment of bone defects.