Translation termination in pyrrolysine-utilizing archaea
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- 作者：Elena Alkalaeva ; Boris Eliseev ; Alex ; re Ambrogelly ; Peter Vlasov ; Fyodor A. Kondrashov ; Sharath Gundllapalli ; Lyudmila Frolova ; Dieter Sö ; ll ; Lev Kisselev
- 关键词：Translation termination ; Archeon ; Pyrrolysine-utilizing archea ; aRF1 ; Polypeptide release factor
- 刊名：FEBS Letters
- 出版年：2009
- 出版时间：3 November 2009
- 年：2009
- 卷：583
- 期：21
- 页码：3455-3460
- 全文大小：855 K
文摘
Although some data link archaeal and eukaryotic translation, the overall mechanism of protein synthesis in archaea remains largely obscure. Both archaeal (aRF1) and eukaryotic (eRF1) single release factors recognize all three stop codons. The archaeal genus Methanosarcinaceae contains two aRF1 homologs, and also uses the UAG stop to encode the 22nd amino acid, pyrrolysine. Here we provide an analysis of the last stage of archaeal translation in pyrrolysine-utilizing species. We demonstrated that only one of two Methanosarcina barkeri aRF1 homologs possesses activity and recognizes all three stop codons. The second aRF1 homolog may have another unknown function. The mechanism of pyrrolysine incorporation in the Methanosarcinaceae is discussed.