Improvement in fruiting body yield by introduction of the Ampullaria crossean multi-functional cellulase gene into Volvariella volvacea
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- 作者：Feng-Yun Zhao ; Jun-Fang Lin ; Xian-Lu Zeng ; Li-Qiong Guo ; Yi-Hong Wang ; Li-Rong You
- 关键词：Volvariella volvacea ; Multi-functional cellulase gene ; Co-transformation ; Biological efficiency ; Fruiting body yields
- 刊名：Bioresource Technology
- 出版年：2010
- 出版时间：August 2010
- 年：2010
- 卷：101
- 期：16
- 页码：6482-6486
- 全文大小：263 K
文摘
The multi-functional cellulase gene, mfc, from Ampullaria crossean was transformed into Volvariella volvacea by PEG-mediated protoplast transformation to improve the biological efficiency and fruiting body yield. PCR and Southern blotting indicated that mfc was integrated into the genomes of four transformants. In laboratory and large scale cultivation experiments, the average biological efficiency of the transformants was 18.39 ± 1.27 % and 27.84 ± 3.21 % , respectively, considerably higher than the corresponding values for untransformed controls of 12.69 ± 1.31 % and 20.63 ± 2.59 % . This is the first report of an improvement in biological efficiency and fruiting body yield of V. volvacea through transgenesis.