Breeding cold tolerance strain by chemical mutagenesis in Volvariella volvacea

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• 作者：Zhu Liu^a ; ^b ; ¹ ; Kai Zhang^a ; ¹ ; Jun-Fang Lin^a ; ^c ; Li-Qiong Guo^a ; ^c ; ^{liqiongguo2009@yahoo.com.cn"" rel=""nofollow} ; ^{guolq@scau.edu.cn"" rel=""nofollow}
• 关键词：Volvariella volvacea ; Basidiospore ; Gill ; Ethyl methane sulfonate ; Diethyl sulfate
• 刊名：Scientia Horticulturae
• 出版年：2011
• 出版时间：26 August 2011
• 年：2011
• 卷：130
• 期：1
• 页码：18-24
• 全文大小：557 K

文摘

Volvariella volvacea is a mushroom well-adapted to high temperature. It optimally grows at 30–35 °C. To breed cold-tolerant strains to expand cultivation region and season, the basidiospores and gills of V. volvacea were treated with chemical mutagens, ethyl methane sulfonate (EMS) and diethyl sulfate (DES), respectively. Two cold-tolerant strains, strains Em-16 and Em-18, were successfully obtained from EMS mutagenesis of gills through 0 °C screening, colony morphology screening and fruiting screening. The biological efficiencies of Em-16 and Em-18 strains were 24.55 % and 23.61 % in the first flush at 27 °C and their biological efficiencies were 46.1 % and 40.5 % higher than the control strain V41, respectively. Their fruiting bodies had a longer storage life at 16 °C, compared with the control strain V41. Amplified fragment length polymorphism analysis shows that the strains Em-16 and Em-18 are new strains.