Genes encoding FAD-binding proteins in Volvariella volvacea exhibit differential expression in homokaryons and heterokaryons

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• 作者：Li Meng ; Junjie Yan ; Baogui Xie ; Yu Li ; Bingzhi Chen ; Shuyan Liu ; Dan Li ; Zhiyun Yang ; Xiancheng Zeng ; Youjin Deng ; Yuji Jiang
• 关键词：Differential expression ; Volvariella volvacea ; Homokaryon ; Heterokaryon ; FAD-binding protein
• 刊名：Microbiological Research
• 出版年：2013
• 出版时间：1 October, 2013
• 年：2013
• 卷：168
• 期：8
• 页码：533-546
• 全文大小：1862 K

文摘

Flavin adenine dinucleotide (FAD)-binding proteins play a vital role in energy transfer and utilization during fungal growth and mycelia aggregation. We sequenced the genome of Volvariella volvacea, an economically important edible fungus, and discovered 41 genes encoding FAD-binding proteins. Gene expression profiles revealed that the expression levels of four distinctly differentially expressed genes in heterokaryotic strain H1521 were higher than in homokaryotic strains PYd15 and PYd21 combined. These observations were validated by quantitative real-time PCR. The results suggest that the differential expression of FAD-binding proteins may be important in revealing the distinction between homokaryons and heterokaryons on the basis of FAD-binding protein functionality.