Expression, purification and characterization of a full-length recombinant HIV-1 Vpu from inclusion bodies
详细信息 查看全文
- 作者：Zikhona Njengelea ; b ; Ronel Kleynhansa ; Yasien Sayedb ; Salerwe Mosebia ; salerwem@mintek.co.za" class="auth_mail" title="E-mail the corresponding author
- 关键词：HIV-1 ; Vpu ; Over-expression ; Solubilization ; Purification ; Refolding
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：109-114
- 全文大小：646 K
文摘
- •
Full-length HIV-1 Vpu was expressed as inclusion bodies in Escherichia coli.
- •
Sufficient amount of pure protein was obtained from a two-step purification process.
- •
The full-length HIV-1 Vpu was purified to ≥95% purity.
- •
The purified recombinant HIV-1 Vpu was found to be functional and correctly folded.