Use of chemostat data for modelling extracellular-inulinase production by Kluyveromyces marxianus in a high-cell-density fed-batch process

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• 作者：Marco C.M.  ; Hensing¹ ; Johannes S.  ; Vrouwenvelder¹ ; Chris  ; Hellinga² ; Johannes P.  ; Van Dijken¹ ; Jack T.  ; Pronk  ; ;   ; ¹
• 关键词：modelling ; chemostat ; extracellular inulinase ; Kluyveromyces marxianus ; fed-batch process
• 刊名：Journal of Fermentation and Bioengineering
• 出版年：1995
• 出版时间：1995
• 年：1995
• 卷：79
• 期：1
• 页码：54-58
• 全文大小：635 K

文摘

Production of extracellular inulinase by low-cell-density (2 kg dry weight¡¤m^?) sucrose-limited chemostat cultures of Kluyveromyces marxianus obeyed saturated kinetics at dilution rates ranging from 0.02 to 0.5 h^?. A non-structured Monod-type equation, describing the relation between specific growth rate and specific extracellular-inulinase production rate, was used to fit experimental data. THis equation was subsequently incorporated in a model for the production of biomass and extracellular inulinase in a high-cell-density (> 100 kg dry weight¡¤m^?) fed-batchculture of K. marxianus grown on sucrose. The model adequately described biomass production in the fed-batch culture. However, the production of extracellular inulinase in the fed-batch process was slightly higher than predicted by the model. This observation may be related to differences in growth conditions between in the chemostat and fed-batch cultures.