Massively parallel sequencing of 17 commonly used forensic autosomal STRs and amelogenin with small amplicons
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- 作者：Eun Hye Kima ; b ; Hwan Young Leeb ; In Seok Yangb ; Sang-Eun Jungb ; Woo Ick Yangb ; Kyoung-Jin Shina ; b ; kjshin@yuhs.ac" class="auth_mail" title="E-mail the corresponding author
- 关键词：Next-generation sequencing ; Autosomal STR ; Small-sized amplicon ; Sequence variation ; Mixture ; Degraded DNA
- 刊名：Forensic Science International: Genetics
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：22
- 期：Complete
- 页码：1-7
- 全文大小：634 K
文摘
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The multiplex PCR system produces small-sized amplicons for 17 forensic STRs and amelogenin.
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STR genotyping results obtained from the MiSeq showed full concordance with those from CE.
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Sequence variations were detected in D2S1338, D3S1358, D8S1179, D21S11 and vWA.
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Successful profiles could be obtained with mixtures up to a 1:19 ratio using NGS analysis.
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This system will be useful to obtain full STR profiles from degraded DNA samples using NGS.