Nanometer-grooved topography stimulates trabecular bone regeneration around a concave implant in a rat femoral medulla model

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• 作者：Alexey Klymov ; MSc^a ; Joost te Riet ; PhD^b ; Peter Mulder^c ; Johannes G.E. Gardeniers ; PhD^d ; John A. Jansen ; DDS ; PhD^a ; X. Frank Walboomers ; PhD^a ; ^{Frank.Walboomers@radboudumc.nl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Nanotopography ; Nanogrooves ; Bone regeneration ; Rat femoral condyle ; Bone implants
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：12
• 期：8
• 页码：2283-2290
• 全文大小：1484 K

文摘

In the present study, a method was developed to reproduce two nanogrooved patterns (groove width/ridge width/depth: 150/150/50 nm and 200/800/70 nm) into cylindrical epoxy resin implants, which were subsequently coated with 20 nm of titanium. Also, implants with a conventional surface roughness (R_q = 1.6 μm) were produced. After cytocompatibility analysis of the produced surfaces, implants were installed into the femoral condyle of rats for 4 and 8 weeks. The histomorphometrical analysis of bone volume in a 100 μm wide zone close to the implant surface showed that only for the 200/800 grooves the amount of bone increased significantly between 4 and 8 weeks of implantation. In addition, at the late time point only implants with the 200/800 pattern revealed a significantly higher bone volume compared to the rough controls. In conclusion, the 200/800 grooved pattern can positively influence bone volume adjacent to the implant surface, and should be evaluated and optimized in further (pre-)clinical studies.