First localisation of somatostatin sst₄ receptor protein in selected human brain areas: an immunohistochemical study

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• 作者：Selmer ; Inger-Sofie ; Schindler ; Marcus ; Humphrey ; Patrick P.A. ; Waldvogel ; Henry J. ; Faull ; Richard L.M. ; et. al.
• 关键词：Neurotransmitters ; modulators ; transporters ; and receptors ; Regional localization of receptors and transmitters ; Immunohistochemistry ; Somatostatin receptor ; Human brain ; Localisation ; GABA ; γ ; -amino butyric acid ; EDTA ; ethylene-diaminetetraacetic aci
• 刊名：Molecular Brain Research
• 出版年：2000
• 出版时间：October 20, 2000
• 年：2000
• 卷：82
• 期：1-2
• 页码：114-125
• 全文大小：2.32 M

文摘

Somatostatin is known to have diverse neurophysiological effects in the mammalian CNS. To date, genes for five different receptors, termed sst_1–5, have been isolated. Recently several reports have been published on the localisation of the individual receptor protein in the rat CNS, but their localisation in the human CNS remains largely unknown. Until now little information about the function of the sst₄ receptor is available, and there is a lack of receptor specific agonists and antagonists. Here, we report for the first time the immunohistochemical localisation of the sst₄ receptor in selected human brain areas using an anti-peptide antibody raised against a carboxy-terminal portion of the receptor protein. Strong receptor immunoreactivity was found in several brain regions, including the hippocampal formation, the cerebellar cortex and the medulla. Further immunohistochemical labelling was observed in the cerebral cortex, the red nucleus and the globus pallidus. Somatodendritic as well as axonal staining was observed. Specific signals were entirely absent following antibody pre-adsorption with the synthetic peptide. The results are in good agreement with the previously published immunohistochemical localisation of the sst₄ receptor in the rat brain. This is the first immunohistochemical study of the localisation of the sst₄ receptor in the human brain, and implicates this receptor in the function of higher centres of the human nervous system.