Activation of CD80 and CD86 molecules on DCs by NC8-pSIP409-3M2e-Fc. IFN-γ+ producing CD8+ T cells in the MLN were induced by the NC8-pSIP409-3M2e-Fc in mice. Total IgA and IgA+B220+ cells in PP and MLN cells were significantly induced by the NC8-pSIP409-3M2e-Fc in mice. Specific sIgA and activated B cell in the growth center of PP were induced by the NC8-pSIP409-3M2e-Fc in mice. NC8-pSIP409-3M2e-Fc provided 60% and 70% protection against the A/Puerto Rico/8/34(H1N1) and mH9N2 AIV, respectively.