Pim-1 is up-regulated by shear stress and is involved in shear stress-induced proliferation of rat mesenchymal stem cells

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• 作者：Ming-Li Tu^a ; ^b ; ¹ ; Han-Qin Wang^c ; ^d ; ¹ ; ^{hanqinwang111@yahoo.com.cn} ; Xiao-Dong Sun^e ; Long-Ju Chen^d ; Xing-Chun Peng^d ; Ya-Hong Yuan^c ; Rui-Ming Li^c ; Xu-zhi Ruan^c ; Dong-Sheng Li^c ; Yong-Jian Xu^a ; ^{xuyongjian66@163.com} ; Zun-Ji Ke^d
• 关键词：Pim-1 ; Shear stress ; Bone ; Marrow ; Proliferation
• 刊名：Life Sciences
• 出版年：2011
• 出版时间：31 January 2011
• 年：2011
• 卷：88
• 期：5-6
• 页码：233-238
• 全文大小：490 K

文摘

Aims

Investigation of the response of mesenchymal stem cells (MSCs) to vascular mechanical forces is very important in the field of cardiovascular intervention. Ser/Thr-protein kinase Pim-1 is a novel transducer of cell survival and the cell cycle that promotes signals in the hematopoietic cell system. Current studies aim to foster an understanding of Pim-1 expression and regulation in MSCs in response to different durations and strengths of laminar shear stress (SS) and to investigate the role of Pim-1 in SS-induced cell proliferation.

Main methods

A parallel-plate flow chamber was used to control the strength and duration of SS. Proliferation was measured with the BrdU cell proliferation assay. The expressions of Pim-1 mRNA and protein were evaluated by reverse transcription-polymerase chain reaction and western blotting, respectively. RNA interference was used to knock down the Pim-1 gene.

Key findings

The results showed that SS up-regulation of Pim-1 mRNA and protein was time-dependent. Pim-1 induction was SS strength-dependent, and the expression level reached a maximum at 30 dynes/cm². Inhibitors of p38MAPK and ERK attenuated the SS-induced expression of Pim-1. In addition, SS significantly increased BrdU-uptake, which was effectively blocked by the silencing of Pim-1.

Significance

These results demonstrated that Pim-1 is expressed in MSCs and plays an important role in the SS-induced proliferation of MSCs.