Enhanced tolerance to NaCl and LiCl stresses by over-expressing Caragana korshinskii sodium/proton exchanger 1 (CkNHX1) and the hydrophilic C terminus is required for the activity of CkNHX1 in Atsos3-1 mutant and yeast

详细信息    查看全文

• 作者：Da-Hai Yang^a ; ^c ; ¹ ; ^{gresea_young@hotmail.com} ; Li-Ying Song^a ; ¹ ; ^{lysong@genetics.ac.cn} ; Jun Hu^a ; ^{jhu@genetics.ac.cn} ; Wei-Bo Yin^a ; ^{wbyin@genetics.ac.cn} ; Zhi-Guo Li^a ; ^{gzhi@genetics.ac.cn} ; Yu-Hong Chen^a ; ^{yhchen@genetics.ac.cn} ; Xiao-Hua Su^b ; ^{suxh@caf.ac.cn} ; Richard R.-C. Wang^d ; ^{Richard.Wang@ARS.USDA.GOV} ; Zan-Min Hu^a ; ^{zmhu@genetics.ac.cn}
• 关键词：Salt tolerance ; NHX ; SOS ; Antiporter ; Exchanger ; Caragana korshinskii
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：13 January 2012
• 年：2012
• 卷：417
• 期：2
• 页码：732-737
• 全文大小：693 K

文摘

Sodium/proton exchangers (NHX antiporters) play important roles in plant responses to salt stress. Previous research showed that hydrophilic C-terminal region of Arabidopsis AtNHX1 negatively regulates the Na⁺/H⁺ transporting activity. In this study, CkNHX1 were isolated from Caragana korshinskii, a pea shrub with high tolerance to salt, drought, and cold stresses. Transcripts of CkNHX1 were detected predominantly in roots, and were significantly induced by NaCl stress in stems. Transgenic yeast and Arabidopsis thaliana sos3-1 (Atsos3-1) mutant over-expressing CkNHX1 and its hydrophilic C terminus-truncated derivative, CkNHX1-¦¤C, were generated and subjected to NaCl and LiCl stresses. Expression of CkNHX1 significantly enhanced the resistance to NaCl and LiCl stresses in yeast and Atsos3-1 mutant. Whereas, compared with expression of CkNHX1, the expression of CkNHX1-¦¤C had much less effect on NaCl tolerance in Atsos3-1 and LiCl tolerance in yeast and Atsos3-1. All together, these results suggest that the predominant expression of CkNHX1 in roots might contribute to keep C. korshinskii adapting to the high salt condition in this plant¡¯s living environment; CkNHX1 could recover the phenotype of Atsos3-1 mutant; and the hydrophilic C-terminal region of CkNHX1 should be required for Na⁺/H⁺ and Li⁺/H⁺ exchanging activity of CkNHX1.