Dopamine D1 receptor (DRD1) 5′ region haplotypes significantly affect transcriptional activity in vitro
详细信息 查看全文
- 作者：Jun Yao ; Mei Ding ; Hao Pang ; Jia-Xin Xing ; Jin-Feng Xuan ; Bao-Jie Wang ; wangbj77@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DRD1 ; Promoter ; Gene polymorphism ; Transcriptional activity ; Schizophrenia
- 刊名：Neuroscience Letters
- 出版年：2015
- 出版时间：16 November 2015
- 年：2015
- 卷：609
- 期：Complete
- 页码：120-123
- 全文大小：630 K
文摘
- •
A fragment spanning −1990 to +10 of DRD1 was amplified to confirm and elucidate the haplotype structure for 5′ promoter region.
- •
Normalized luciferase activities of nine cloned haplotypes (H2–H9) were compared with the common haplotype (H1) after transfecting SH-SY5Y and HEK293 cells.
- •
In SH-SY5Y cell, there were significant differences between H2–H9 and H1, respectively.
- •
In HEK293 cell, no significant difference was observed among them except H8.