Chromatography-based methods for determining molar extinction coefficients of cytotoxic payload drugs and drug antibody ratios of antibody drug conjugates
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- 作者：Chunlei Wang ; chunlei.wang@bms.com" class="auth_mail" title="E-mail the corresponding author ; Sike Chen ; Janet Caceres-Cortes ; Richard Y.-C. Huang ; Adrienne A. Tymiak ; Yingru Zhang
- 关键词：Molar extinction coefficient (MEC) ; Drug antibody ratio (DAR) ; Chemiluminescence nitrogen detector (CLND) ; Antibody drug conjugate (ADC) ; UV spectrophotometry
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：1455
- 期：Complete
- 页码：133-139
- 全文大小：641 K
文摘
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An HPLC method with dual detectors was developed to measure the extinction coefficients.
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The MEC was solved by Beer’s law (A = εcl), with A and c obtained from DAD and CLND.
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The method measures MEC accurately even when the sample is impure or in a mixture.
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Compared to the only available method, our method is sample sparing and easy to implement.
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The MEC has been successfully used for DAR characterization of ADCs in drug discovery.