Preparing tauroursodeoxycholic acid (TUDCA) using a double-enzyme-coupled system
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- 作者：Qingzhi Ji<sup>asup> ; Jun Tan<sup>asup><sup> ; sup><sup>bsup><sup> ; sup><sup>tanjunmail@126.com" class="auth_mail" title="E-mail the corresponding authorsup> ; Liancai Zhu<sup>asup> ; Deshuai Lou<sup>asup> ; Bochu Wang<sup>asup><sup> ; sup><sup>wangbc2000@126.com" class="auth_mail" title="E-mail the corresponding authorsup>
- 关键词：Immobilized enzyme ; Enzyme biocatalysis ; Biotransformations ; Enzyme activity ; 7伪-hydroxysteroid dehydrogenases ; 7尾-hydroxysteroid dehydrogenases ; Bile acid
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：105
- 期：part_PA
- 页码：1-9
- 全文大小：1249 K
文摘
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TUDCA was prepared from TCDCA in vitro using the double-enzyme-coupled system.
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7伪- and 7尾-HSDH, was fixed by separate immobilization and co-immobilization.
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Coenzyme NADP<sup>+sup> regeneration was achieved by the double-enzyme-coupled system.
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The double-enzyme-coupled system is promissing for the high efficient preparation of TUDCA in vitro.