A colorimetric biosensor using Fe₃O₄ nanoparticles for highly sensitive and selective detection of tetracyclines

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• 作者：Yilin Wang^a ; ^bAuthor Vitae ; Yujing Sun^aAuthor Vitae ; Haichao Dai^a ; ^bAuthor Vitae ; Pengjuan Ni^a ; ^bAuthor Vitae ; Shu Jiang^a ; ^bAuthor Vitae ; Wangdong Lu^a ; ^bAuthor Vitae ; Zhen Li^a ; ^bAuthor Vitae ; Zhuang Li^a ; ^{zli@ciac.ac.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Fe3O4 magnetic nanoparticles ; Peroxidase-like activity ; Tetracyclines ; 3 ; 3&prime ; 5 ; 5&prime ; -tetramethylbenzidine ; UV&ndash ; vis
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：29 November 2016
• 年：2016
• 卷：236
• 期：Complete
• 页码：621-626
• 全文大小：1372 K

文摘

A novel colorimetric biosensor for the determination of tetracyclines (TCs) based on the Fe₃O₄ magnetic nanoparticles (Fe₃O₄ MNPs) is presented in this paper. TCs are known to have a strong tendency to complex with metal ions such as Fe(II) and Fe(III) on the surface of Fe₃O₄ MNPs. Experiments results show that the complexation of Fe₃O₄ MNPs and TCs could lead to the accelerated catalysing H₂O₂-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by Fenton chemistry. The reaction starts with oxidation of Fe₃O₄ MNPs-TCs complex by dissolved oxygen to generate reactive oxygen species (ROS), primarily ·OH, leading to deepen the color of the solution. Analysis results can be seen with the naked eye and monitored by UV–vis spectra. Under the optimal conditions, the detection limits are 26 nM for oxytetracycline (OTC), 45 nM for tetracycline (TC) and 48 nM for doxycycline (DOX). Moreover, this method has been applied for the determination of OTC content in drugs with satisfactory results. The proposed colorimetric biosensor is advantageous over other conventional methods in terms of convenient operation without any complicated chemical synthesis, modification, or tedious experimental procedures and its simple signal generation and detection by the naked eye. To the best of our knowledge, this is the first report on the enhancement effect of TCs on Fe₃O₄ MNPs-TMB-H₂O₂ reaction and its application in colorimetric TCs analysis. The designed colorimetric strategy may provide a promising alternative detection for TCs in clinical samples.