A simple and low-cost cotton thread based nucleic acid biosensor with temperature-dependent pattern (pCTNAB) was developed.

Liquid wax with tunable state was used to fabricate the pattern to enrich the capture molecular on a narrow test zone.

The sample wicked faster at the CTNAB and less sample was needed compared to the previous reported lateral flow strip.

The proposed sensor showed fine sensitivity, reproducibility and excellent anti-interference ability.