Infectious cDNA clones of four viroids in Coleus blumei and molecular characterization of their progeny

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• 作者：Dongmei Jiang ; Rui Gao ; Lv Qin ; Zujian Wu ; Lianhui Xie ; Wanying Hou ; Shifang Li
• 关键词：Viroid ; Coleus blumei ; Infectious clone ; Host
• 刊名：Virus Research
• 出版年：13 February, 2014
• 年：2014
• 卷：180
• 期：Complete
• 页码：97-101
• 全文大小：1132 K

文摘

Four viroid species infecting Coleus blumei, named Coleus blumei viroid 1-4 (CbVd-1-CbVd-4), and two tentative new viroid species, named CbVd-5 and CbVd-6, have been characterized, for two of which (CbVd-5 and CbVd-6, first reported in 2009), there is no established bioassay. Here, infectious clones were used as inoculums sources and the biological properties of CbVd-1, -3, -5 and -6 were assessed. When dimeric CbVd (+) RNAs synthesized in vitro were bioassayed, the first detection time for the four CbVds was different, ranging from 45 to 300 days. In addition, we confirmed that CbVd-5 and CbVd-6 can be transmitted via seeds. Molecular characterization of their progeny from slash-inoculated plants one year after inoculation demonstrated that the genetic diversity of CbVd populations may depend on the infected coleus plants and on the viroid genotype.