Thermal and clinical performance of a closed device designed for human oocyte vitrification based on the optimization of the warming rate

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• 作者：Miguel Gallardo^a ; ^b ; Marí ; a Hebles^b ; Beatriz Migueles^b ; Mó ; nica Dorado^b ; Laura Aguilera^b ; Mercedes Gonzá ; lez^b ; Paloma Piqueras^b ; Lorena Montero^b ; Pascual Sá ; nchez-Martí ; n^b ; Fernando Sá ; nchez-Martí ; n^b ; Ramó ; n Risco^a ; ^c ; ^{risco@us.es" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Vitrification ; Closed system ; Aseptic ; Human ; Donor oocytes ; Warming rate
• 刊名：Cryobiology
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：73
• 期：1
• 页码：40-46
• 全文大小：820 K

文摘

Although it was qualitatively pointed out by Fahy et al. (1984), the key role of the warming rates in non-equillibrium vitrification has only recently been quantitatively established for murine oocytes by Mazur and Seki (2011). In this work we study the performance of a closed vitrification device designed under the new paradigm, for the vitrification of human oocytes.

The vitrification carrier consists of a main straw in which a specifically designed capillary is mounted and where the oocytes are loaded by aspiration. It can be hermetically sealed before immersion in liquid nitrogen for vitrification, and it is warmed in a sterile water bath at 37 °C. Measured warming rates achieved with this design were of 600.000 ºC/min for a standard DMEM solution and 200.000 ºC/min with the vitrification solution for human oocytes.

A cohort of 143 donor MII sibling human oocytes was split into two groups: control (fresh) and vitrified with SafeSpeed device. Similar results were found in both groups: survival (97.1%), fertilization after ICSI (74.7% in control vs. 77.3% in vitrified) and good quality embryos at day three (54.3% in control vs. 58.1% in vitrified) were settled as performance indicators. The pregnancy rate was 3/6 (50%) for the control, 2/3 (66%) for vitrified and 4/5 (80%) for mixed transfers.