A fast solution switching system with temperature control for single cell measurements

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• 作者：Duk-Su Koh^a ; ^b ; ^{koh@u.washington.edu"" rel=""nofollow} ; Liangyi Chen^a ; ¹ ; ^{lychen@pku.edu.cn"" rel=""nofollow} ; Carmen A. Ufret-Vincenty^a ; ² ; ^{cufret@u.washington.edu"" rel=""nofollow} ; Seung-Ryoung Jung^a ; ³ ; ^{jungsr@gmail.com"" rel=""nofollow}
• 关键词：Solution switch ; Temperature control ; Ca²⁺ measurement ; Amperometry ; Perfusion exchange system
• 刊名：Journal of Neuroscience Methods
• 出版年：2011
• 出版时间：15 July 2011
• 年：2011
• 卷：199
• 期：1
• 页码：35-42
• 全文大小：842 K

文摘

This article describes a perfusion system for biophysical single cell experiments at the physiological temperature. Our system regulates temperature of test solutions using a small heat exchanger that includes several capillaries. Water circulating inside the heat exchanger warms or cools test solutions flowing inside the capillaries. Temperature-controlled solutions are delivered directly to a single cell(s) through a multibarreled manifold that switches solutions bathing a cell in less than 1 s. This solution exchange is optimal for patch clamp, single-cell microamperometry, and microfluorometry experiments. Using this system, we demonstrate that exocytosis from pancreatic β cells and activation of TRPV1 channels are temperature sensitive. We also discuss how to measure local temperature near a single cell under investigation.