Polymorphisms of human platelet alloantigens HPA-1 and HPA-2 associated with severe coronary artery disease

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• 作者：Nesrine Abboud ; Haitham Amin ; Lakdhar Ghazouani ; Sonia Ben Haj Khalifa ; Ali Ben Khalafallah ; Fawzi Aded ; Wassim Y. Almawi ; Touhami Mahjoub
• 关键词：Human platelet alloantigens ; PCR ; Gene polymorphism ; Coronary artery disease
• 刊名：Cardiovascular Pathology
• 出版年：2010
• 出版时间：September-October 2010
• 年：2010
• 卷：19
• 期：5
• 页码：302-307
• 全文大小：126 K

文摘

Objectives

Insofar as platelet membrane glycoprotein (GP) polymorphisms were identified as potential risk factors for coronary artery disease (CAD), we investigated the contribution of human platelet antigen (HPA)-1 (GPIIb/IIIa) and HPA-2 (GPIb/IX) alleles and haplotypes to CAD pathogenesis.

Methods

Study subjects comprised 247 middle-age CAD patients and 316 age-, gender-, and race-matched controls; HPA genotyping was performed by polymerase chain reaction with sequence specific primers.

Results

The frequencies of HPA-1b (P<.001) and HPA-2b (P<.001) alleles and HPA-1a/1b (P<.001), HPA-1b/1b (P<.001), and HPA-2a/2b (P<.001) genotypes were higher in patients than control subjects. Select HPA haplotypes comprising the HPA-1b/2a (Pc=2.2×10⁻⁴) and HPA-1b/2b (Pc=.001) haplotypes which were positively associated, and the HPA-1a/2a (Pc=3.2×10⁻⁵) which was negatively associated with CAD, confer a disease susceptibility and protective nature to these haplotypes. Multivariate analysis confirmed the positive association of HPA-1b/2a [adjusted odds ratio (aOR)=3.63; 95 % CI=2.42–5.43] and HPA-1b/2b (aOR=2.92; 95 % CI=1.43–5.94) haplotypes with CAD, after adjustment for a number of covariates.

Conclusions

Our results suggest that HPA-1/HPA-2 haplotypes may be considered to be a major risk factor for CAD in middle-aged Tunisians.