Antiviral and antiproliferative effects of canine interferon-位1

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• 作者：Tomonori Ichihashi ; Atsushi Asano ; Tatsufumi Usui ; Takashi Takeuchi ; Yasuko Watanabe ; Yoshiaki Yamano
• 关键词：Antiviral activity ; Antiproliferation ; Dog ; Interferon-stimulated genes ; IFN-位1
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：15 November, 2013
• 年：2013
• 卷：156
• 期：1-2
• 页码：141-146
• 全文大小：694 K

文摘

Interferon (IFN)-位s, members of the type III IFN group, were recently identified in several vertebrates. Although IFN-位s have the potential to be utilized as antiviral and antitumor agents in veterinary medicine, the biological properties of IFN-位s have not yet been studied in companion animals. In this study, we analyzed the expression of canine IFN-位s and their receptors, produced a recombinant canine IFN-位1 protein, and investigated its antiviral and antiproliferative activities using a canine kidney epithelial cell line, MDCK cells. MDCK cells were found to express type III IFN molecules, IFN-位1 and IFN-位3, and the receptors, IFN位R1 and IL10R2. IFN-位1 was induced faster than IFN-位3 by stimulation with poly (I:C). His-tagged IFN-位1 protein expressed in Escherichia coli inhibited cytolytic plaque formation by influenza A virus infection, and induced the expression of interferon-stimulated genes, Mx1 and OAS1, in MDCK cells. In addition, recombinant IFN-位1 inhibited the proliferation of MDCK cells slightly. These effects were observed in a dose-dependent manner. These results indicate that canine IFN-位1 has antiviral effect, and suggest the potential applicability of canine IFN-位1 as a therapeutic agent.