In vitro reconstitution and biochemical analyses of the Schizosaccharomyces pombe nucleosome

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• 作者：Masako Koyama^a ; Wataru Nagakura^a ; Hiroki Tanaka^a ; Tomoya Kujirai^a ; Yuji Chikashige^b ; Tokuko Haraguchi^b ; ^c ; Yasushi Hiraoka^b ; ^c ; Hitoshi Kurumizaka^a ; ^d ; ^e ; ^{kurumizaka@waseda.jp}
• 关键词：Schizosaccharomyces pombe ; Histones ; Nucleosome ; Reconstitution ; Thermal stability ; Micrococcal nuclease
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：896-901
• 全文大小：1458 K
• 卷排序：482

文摘

S. pombe histones were purified as recombinant proteins. The recombinant S. pombe histones efficiently form nucleosomes in vitro. The S. pombe nucleosome has distinct stability and DNA dynamics.