Optimization of calcium concentration of saliva with phosphoryl oligosaccharides of calcium (POs-Ca) for enamel remineralization in vitro

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• 作者：Tomoko Tanaka^a ; Takatsugu Kobayashi^a ; ^{kobayashi-takatsugu@glico.co.jp} ; Hiroshi Takii^a ; Hiroshi Kamasaka^a ; Noboru Ohta^b ; Tatsuhito Matsuo^b ; ¹ ; Naoto Yagi^b ; Takashi Kuriki^a
• 关键词：Phosphoryl oligosaccharides ; Calcium ; Remineralization ; Enamel ; Hydroxyapatite ; X-ray diffraction ; Crystal
• 刊名：Archives of Oral Biology
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：58
• 期：2
• 页码：174-180
• 全文大小：582 K

文摘

Objective

Phosphoryl oligosaccharides of calcium (POs-Ca) are highly soluble calcium source made from potato starch. The aim of this study was to investigate the optimal concentrations of POs-Ca for the remineralization of subsurface enamel lesions in vitro.

Design

Demineralized bovine enamel slabs (n = 5) were remineralized in vitro for 24 h at 37 ¡ãC with artificial saliva (AS) containing 0-0.74 % POs-Ca to adjust the Ca/P ratio to 0.4-3.0, then sectioned and analysed by transversal microradiography (TMR). The data were analysed by Scheffe's post hoc test. The Ca/P ratio with most remineralization was used to investigate the effect of calcium on enamel remineralization (n = 11). The demineralized slabs were treated with AS with calcium-chloride- (CaCl₂-) or POs-Ca with an identical calcium content, and sectioned for TMR and wide-angle X-ray diffraction (WAXRD) analyses to evaluate the local changes in hydroxyapatite (HAp) crystal content. The data were analysed using the Mann-Whitney U-test.

Results

The highest mineral recovery rate resulted from addition of POs-Ca to adjust the Ca/P to 1.67. At this ratio, the mineral recovery rate for AS containing POs-Ca (24.2 ¡À 7.4 % ) was significantly higher than that for AS containing CaCl₂ (12.5 ¡À 11.3 % ) (mean ¡À SD, p < 0.05). The recovery rate of HAp crystallites for AS containing POs-Ca (35.7 ¡À 10.9 % ) was also significantly higher than that for AS containing CaCl₂ (23.1 ¡À 13.5 % ) (p < 0.05). The restored crystallites were oriented in the same directions as in sound enamel.

Conclusions

POs-Ca effectively enhances enamel remineralization with ordered HAp at a Ca/P ratio of 1.67.