Addition of penicillin-streptomycin into semen was suitable for cryopreservation.
Addition of penicillin-streptomycin (PS) did not affect sperm motility and viability.
Addition of PS reduced bacterial numbers and removed bacterial contaminants.
Post-thawed semen treated with penicillin–gentamicin (PG) had low sperm quality.
Fertilization rate was similar between PS-treated and antibiotic-free semen.