How the rice weevil breaks down the pectin network: Enzymatic synergism and sub-functionalization
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- 作者：Roy Kirscha ; rkirsch@ice.mpg.de" class="auth_mail" title="E-mail the corresponding author ; David G. Heckela ; Yannick Paucheta ; ypauchet@ice.mpg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Plant cell wall degradation ; Polygalacturonase ; Pectin methylesterase ; Weevils ; Horizontal gene transfer
- 刊名：Insect Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：71
- 期：Complete
- 页码：72-82
- 全文大小：1811 K
文摘
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Ten putative pectolytic enzymes from the rice weevil Sitophilus oryzae were heterologously expressed and functionally characterized.
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Activity assays revealed polygalacturonases and pectin methylesterases with different substrate specificities as well as inactive enzymes.
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Polygalacturonases and pectin methylesterases degrade pectin synergistically.
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Lack of substrate binding and amino acid substitutions explain loss of pectolytic activity.