Succinyl-β-cyclodextrin modified gold biochip improved seroimmunological detection sensitivity for Lyme disease

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• 作者：Lei Ye^a ; ¹ ; Na-Li Huang^a ; ¹ ; Yi-Xin Du^b ; Marion Schneider^b ; Wei-Dong Du^a ; ^{weidong.du@ahmu.edu.cn}
• 关键词：Protein biochip ; Cyclodextrin ; Variable major protein-like sequence ; Enzyme-linked immunosorbent assay ; Lyme disease
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：8 February 2017
• 年：2017
• 卷：953
• 期：Complete
• 页码：48-56
• 全文大小：1677 K
• 卷排序：953

文摘

This research developed a succinyl-β-cyclodextrin modification on gold surface. The succinyl-β-cyclodextrin modified gold surface provided impressive effectes. Limit of detectable concentration of VlsE antigen on the modified surface was 0.000475 μg/ml. Protein biochip based approach was comparable with ELISA based assay in detection of anti-VlsE antibody.