A hypochlorous acid turn-on fluorescent probe based on HOCl-promoted oxime oxidation and its application in cell imaging
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- 作者：Shiang-Yi YuAuthor Vitae ; Chen-Yi HsuAuthor Vitae ; Wei-Chieh ChenAuthor Vitae ; Ling-Fang WeiAuthor Vitae ; Shu-Pao Wu ; spwu@mail.nctu.edu.tw" class="auth_mail ; spwu@faculty.nctu.edu.tw" class="auth_mailAuthor Vitae
- 关键词：Hypochlorous acid ; Fluorescent probe ; Coumarin ; Bioimaging
- 刊名：Sensors and Actuators B: Chemical
- 出版年：June, 2014
- 年：2014
- 卷：196
- 期：Complete
- 页码：203-207
- 全文大小：915 K
文摘
A coumarin-based fluorometric probe, HS1, has been successfully developed for highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of oxime. The reaction is accompanied by a 7-fold increase in the fluorescent quantum yield (from 0.03 to 0.21). The fluorescence intensity of the reaction between HOCl and HS1 shows a good linearity in the HOCl concentration range of 1-20 渭M and a low detection limit of 25 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HS1 probe could be an efficient fluorescent probe for HOCl in living cells.