Tanshinone components in S. miltiorrhizae were obtained by ultrasound-assisted extraction.
A UFLC–MS/MS method was developed for simultaneous analysis of these components.
MRM-IDA-EPI mode was used for supplementary characteristic identification.
The validated method gave lower LODs and LOQs for target analytes.
UFLC–MS/MS with MRM-IDA-EPI mode was preferred for other complicated matrices.