Oligodendrocyte progenitor cells derived from mouse embryonic stem cells give rise to type-1 and type-2 astrocytes in vitro

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• 作者：Chen Chen ; Daniel Daugherty ; Peng Jiang ; Wenbin Deng
• 关键词：Oligodendrocyte progenitor cells ; Mouse embryonic stem cells ; Neural differentiation ; Type-1 and type-2 astrocytes
• 刊名：Neuroscience Letters
• 出版年：2012
• 出版时间：15 August, 2012
• 年：2012
• 卷：523
• 期：2
• 页码：180-185
• 全文大小：1552 K

文摘

Oligodendrocyte progenitor cells (OPCs) in primary culture can give rise to mature oligodendrocytes and type-2, but not type-1 astrocytes depending on the culture conditions. The OPCs thus are called oligodendrocyte-type-2 astrocyte (O2-A) progenitor cells. Mouse embryonic stem cells (mESCs) have been efficiently differentiated into OPCs; however, the fate plasticity of mESC-derived OPCs is not well characterized. In the present study, using GFP-Olig2 mESC line, we showed that the Olig2⁺/GFP⁺/A2B5⁺/NG2⁺ OPCs derived from GFP-Olig2 mESCs can mature into oligodendrocytes when co-cultured with mESC-derived neurons. Interestingly, when induced to astrocytic differentiation by bone morphogenetic protein-4, these mESC-derived OPCs can not only generate type-2 astrocytes, but also type-1 astrocytes. These results challenge the dogma that OPCs in culture can only generate type-2, but not type-1 astrocytes, and support the in vivo finding that during perinatal development, OPCs can give rise to a subset of type-1 astrocytes.