Cyclic interconversion of methionine containing peptide between oxidized and reduced phases monitored by reversed-phase HPLC and ESI-MS/MS

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• 作者：Yulong Jin ; Yanyan Huang ; ^{yyhuang@iccas.ac.cn} ; Yunfeng Xie ; Wenbing Hu ; Fuyi Wang ; Guoquan Liu ; Rui Zhao ; ^{zhaorui@iccas.ac.cn}
• 关键词：Methionine ; Peptide ; Oxidation/reduction ; HPLC ; ESI-MS/MS
• 刊名：Talanta
• 出版年：2012
• 出版时间：30 January, 2012
• 年：2012
• 卷：89
• 期：Complete
• 页码：531-536
• 全文大小：633 K

文摘

The cyclic oxidation and reduction of methionine (Met) containing peptides and proteins play important roles in biological system. This work was contributed to analysis the cyclic oxidation and reduction processes of a methionine containing peptide which is very likely to relate in the cell signal transduction pathways. To mimic the biological oxidation condition, hydrogen peroxide was used as the reactive oxygen species to oxidize the peptide. Reversed-phase high-performance liquid chromatography and mass spectrometry were employed to monitor the reactions and characterize the structural changes of the products. A rapid reduction procedure was developed by simply using KI as the reductant, which is green and highly efficient. By investigation of the cyclic oxidation and reduction process, our work provides a new perspective to study the function and mechanism of Met containing peptides and proteins during cell signaling processes as well as diseases.