Regulation of cell proliferation and migration in gallbladder cancer by zinc finger X-chromosomal protein
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- 作者：Zhujun Tan ; Shenglai Zhang ; Maolan Li ; Xiangsong Wu ; Hao Weng ; Qian Ding ; Yang Cao ; Runfa Bao ; Yijun Shu ; Jiasheng Mu ; Qichen Ding ; Wenguang Wu ; Jiahua Yang ; Lin Zhang ; Yingbin Liu
- 关键词：GBC ; Gallbladder carcinoma ; ZFX ; Zinc finger X-chromosomal protein ; Lv-shZFX ; ZFX-siRNA-expressing lentivirus ; ZNF ; Zinc finger ; ZBTB20 ; Zinc finger and BTB domain containing 20 ; HCC ; Hepatocellular carcinoma ; ZNF267 ; Zinc finger protein 267 ; KCMF1 ; Potas
- 刊名：Gene
- 出版年：2013
- 出版时间：10 October, 2013
- 年：2013
- 卷：528
- 期：2
- 页码：261-266
- 全文大小：1018 K
文摘
Gallbladder carcinoma (GBC) is one of the mostly aggressive and fatal malignancies. However, little is known about the oncogenic genes that contributed to the development of GBC. Zinc finger X-chromosomal protein (ZFX) was a novel member of the Krueppel C2H2-type zinc-finger protein family and its down-regulation led to impaired cell growth in human laryngeal squamous cell carcinoma. Here, we aim to investigate the function of ZFX in GBC cell proliferation and migration. Loss of function analysis was performed on GBC cell line (GBC-SD) using lentivirus-mediated siRNA against ZFX. The proliferation, in vitro tumorigenesis (colony-formation) ability as well as cell migration was significantly suppressed after GBC-SD cells which were infected with ZFX-siRNA-expressing lentivirus (Lv-shZFX). Our finding suggested that ZFX promoted the growth and migration of GBC cells and could present a potential molecular target for gene therapy of GBC.