A simple capillary zone electrophoretic assay for reserpin using end-column amperometric detection at a carbon fiber micro-disk array electrode

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• 作者：Jin ; Wenrui ; Ye ; Xiaoying ; Yu ; Daiqing ; Dong ; Qian
• 关键词：Electrophoresis ; Electrochemical detection ; Detection ; Electrodes ; Reserpin ; Drug ; Medicine
• 刊名：Analytica Chimica Acta
• 出版年：2000
• 出版时间：March 9, 2000
• 年：2000
• 卷：408
• 期：1-2
• 页码：257-262
• 全文大小：95.5 K

文摘

Capillary zone electrophoresis was employed for the determination of reserpin using an end-column amperometric detection with a carbon fiber micro-disk array electrode at a constant potential of +1.30 V vs. saturated calomel electrode. The optimum conditions of separation and detection are 1.0 × 10⁻⁴ mol/l Tris–8.4 × 10⁻⁵ mol/l HCl for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 8.0 × 10⁻⁷ mol/l or 1.4 fmol (S/N = 2). The relative standard deviation is 2.5 % for the migration time and 1.5 % for the electrophoretic peak current. The method was applied to the determination of reserpin in human serum.