Activation of Cholinergic Anti-Inflammatory Pathway Contributes to the Protective Effects of 100 % Oxygen Inhalation on Zymosan-Induced Generalized Inflammation in Mice

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• 作者：Zishen Zhang ; M.D.^&lowast ; ; ² ; Xiaoguang Bai ; M.D.^&lowast ; ; ² ; Kejun Du ; Ph.D. ; M.D.^&dagger ; ; ² ; Yi Huang ; M.D.^&lowast ; ; ² ; Wenyong Wang ; M.D.^&Dagger ; ; Yiling Zhao ; M.D.^&Dagger ; ; Yuping Pei ; M.D.^&lowast ; ; Jinglan Mu ; M.D.^&lowast ; ; Han Han ; M.D.^&lowast ; ; Sheng Hu ; Ph.D. ; M.D.^&lowast ; ; Shuzhi Li ; M.D.^&lowast ; ; Hailong Dong ; Ph.D. ; M.D.^&lowast ; ; Yan Lu ; Ph.D. ; M.D.^&lowast ; ; Lichao Hou ; Ph.D. ; M.D.^§ ; ; ^{Lichao.Hou@hotmail.com} ; Lize Xiong ; Ph.D. ; M.D.^&lowast ; ; ^{mzkxlz@126.com}
• 关键词：zymosan ; systemic inflammation ; cholinergic anti-inflammatory pathway ; inflammatory cytokine ; 100 % oxygen
• 刊名：Journal of Surgical Research
• 出版年：2012
• 出版时间：15 May, 2012
• 年：2012
• 卷：174
• 期：2
• 页码：e75-e83
• 全文大小：1154 K

文摘

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Background

The 100 % oxygen inhalation has been demonstrated to have a protective effect on mice with zymosan-induced generalized inflammation. However, the underlying mechanism is largely unknown. The present study was designed to explore the role of the cholinergic anti-inflammatory pathway in this animal model.

Methods

Oxygen inhalation was given to mice at 4?and 12 h after zymosan injection. One group of mice?underwent vagotomy 7 d before zymosan injection. The other two groups of mice either received nicotinic acetylcholine receptor (nAChR) antagonist mecamylamine, or ¦Á7 nicotinic acetylcholine receptor (¦Á7nAChR) antagonist methyllycaconitine 30 min before oxygen was given.

Results

The 100 % oxygen treatment significantly decreased the serum level of TNF-¦Á and increased the serum level of IL-10. The pathologic changes of the heart, lung, liver, and kidney were attenuated, as well as the dysfunction of liver and kidney. The 7-d survival rate of zymosan-challenged mice was also improved. Conversely, all these protective effects caused by pure oxygen treatment were abolished in?those animals that received anti-cholinergic treatments.

Conclusions

The cholinergic anti-inflammatory pathway may be involved in the 100 % oxygen protective mechanism against zymosan-induced generalized inflammation in mice.